a comparison of mineral adsorbents zeolite and bentonite vs. organic adsorbent (mycosorb) and mineral – organic adsorbent (biotox) as their regardstheir potential of adsorbing aflatoxin b1

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مجید سواری

دانشجوی کارشناسی ارشد پردیس کشاورزی و منابع طبیعی دانشگاه تهران مهدی دهقان بنادکی

دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران کامران رضایزدی

دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمد جوان¬نیکخواه

دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

mycotoxins are secondary metabolites produced by fungi following their infestation of food crops.one of the effective approaches to control aflatoxicosis is the use of adsorbents. the aim thusfollowed in the present study was a comparison of various adsorbents viz. bentonite, zeolite, vs. anorganic commercial adsorbent (mycosorb) and a mineral – organic commercial adsorbent (biotox)regarding their potential to adsorb aflatoxin b1. afb1- contaminated rice was prepared by itsinoculation with the aspergillus parasiticus, strain type pttc 5286. levels of aflatoxin, present in thecontaminated rice were determined through hplc. afb was chloroform extracted thrice, by havingrice soaked in chloroform. adsorbents were individually mixed at three different ratios with afb(1:1000, 1:5000 and 1:15000, w/w), in a buffer solution, centrifuged and the supernatant evaluated foraflatoxin b conted using aflatoxin b elisa kit. the design employed was a completelyrandomized one of: 4×3 factorial arrangement. results indicated that at 1:15000 aflatoxin toadsorbent ratio, zeolite, mycosorb and biotox sequestered (adsorbed) about 0.8, 0.81 and 0.83 of theafb. this efficacy decreased with the amounts of adsorbents reduced. bentonite had the poorestsequestering efficacy, with 0.38 being the maximum value obtained for the 1:15000 aflatoxin toadsorbent ratio treatment. there was a significant difference observed among the three ratios and aswell between biotox and other adsorbents for their percentages of afb1 adsorption (p

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عنوان ژورنال:
علوم دامی ایران

جلد ۴۴، شماره ۱، صفحات ۱۰۵-۱۱۲

کلمات کلیدی
mycotoxins are secondary metabolites produced by fungi following their infestation of food crops.one of the effective approaches to control aflatoxicosis is the use of adsorbents. the aim thusfollowed in the present study was a comparison of various adsorbents viz. bentonite zeolite vs. anorganic commercial adsorbent (mycosorb) and a mineral – organic commercial adsorbent (biotox)regarding their potential to adsorb aflatoxin b1. afb1 contaminated rice was prepared by itsinoculation with the aspergillus parasiticus strain type pttc 5286. levels of aflatoxin present in thecontaminated rice were determined through hplc. afb was chloroform extracted thrice by havingrice soaked in chloroform. adsorbents were individually mixed at three different ratios with afb(1:1000 1:5000 and 1:15000 w/w) in a buffer solution centrifuged and the supernatant evaluated foraflatoxin b conted using aflatoxin b elisa kit. the design employed was a completelyrandomized one of: 4×3 factorial arrangement. results indicated that at 1:15000 aflatoxin toadsorbent ratio zeolite mycosorb and biotox sequestered (adsorbed) about 0.8 0.81 and 0.83 of theafb. this efficacy decreased with the amounts of adsorbents reduced. bentonite had the poorestsequestering efficacy with 0.38 being the maximum value obtained for the 1:15000 aflatoxin toadsorbent ratio treatment. there was a significant difference observed among the three ratios and aswell between biotox and other adsorbents for their percentages of afb1 adsorption (p

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